Author Archive | Sylvia Janetzki

Elispot Plate Reading Guidelines published in Nature Protocols

The results of the Elispot harmonization efforts of the Cancer Immunotherapy Consortium (CIC/CRI), in collaboration with European Association of Immunotherapy of Cancer (CIMT), have led to guidelines for the evaluation of Elispot plates, based on a large Elispot plate reading panel and consensus process involving more than 100 scientists from all over the world. The

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Another cause for Overnight resting

A group from TPIMS in San Diego revisits the effects of overnight resting on frozen PBMC and their performance in IFNg Elispot. The advantages of including a resting step is clearly demonstrated and confirmed by rigorous statistical testing, using an adapted version of the DFR method for comparing multiple test runs of the same sample.

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Excellent publication about IVS

Fresh of the press comes an excellent publication from Chudley et al. about in vitro stimulation of PBMC, harmonization of IVS protocols, and effects of IVS on results from Elispot and Multimer assays. This paper can be found in Cancer Immunology and Immunotherapy. It is written in full MIATA compliance and hence also listed in

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ICS Gating Panel

While not being an Elispot panel, it is  so important that this announcement made it to the ZellNet site: The CIC/CRI announced their 2nd ICS Gating panel. Participation is free, and is not restricted to membership. More information can be found here.

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