The distribution-free resampling (DFR) method has been specifically developed for response definition in Elispot.

It takes the peculiarities of Elispot data into account which are:

  • Limited data points (hence no assumption about the data distribution can be made)
  • Variability
  • Possible high background reactivity

Important reference:

Z Moodie*, L Price*, C Gouttefangeas, A Mander, S Janetzki, M Lower, MJ Welters, C Ottensmeier, SH van der Burg, CM Britten. Response definition criteria for ELISpot assays revisited. Cancer Immunol Immunother, 59(10):1489-501, 2010. *shared first authorship

An online tool exists to run the DFR yourself. Please follow the instructions including the required data format given there. The page is hosted by Fred Hutch (Institute  Statistical Center for HIV/AIDS Research and Prevention (SCHARP).


Scroll to Top