ZellNet is committed to ensuring your Elispot experience is not only successful but also exceptional and enjoyable. Our unrivaled customer service is always at your disposal, ready to assist you.
We offer help
Starting with the setup of SOPs to the final evaluation of plates and statistical analysis of results, from experimental design of research projects to guidance for clinical trial utilization of Elispot and Fluorospot including assay validation – ZellNet provides help whenever needed.
On-site service
We can conduct a wet workshop in your lab following your experimental requirements and environment to get you started like a Pro. Looking to outsource your assay, maybe also sample handling, preparation and storage? We can help you in your task.
Plate reading service
Don’t want to buy an expensive Plate Reader System? Do you prefer your samples being evaluated in a blinded fashion, removing the hassles of establishing a qualified plate reading SOP and of potential bias? No time for training, updates and upgrades? ZellNet specializes in superior plate reading services for any Elispot and Fluorospot assay and plate format since 1998.
Full overview ZellNet services
Next generation Plate evaluation (IRIS) using RAWSpot technology for Elispot and Fluorospot for up to 4 analytes simultaneously: see our dedicated page
Classic Image analysis (Zeiss KS Elispot Reader) for single and dual color Elispot is also available.
Pricing:
- Single color Elispot plate: $60.00
- Dual color Elispot plate: $150.00
- Single color Fluorospot plate: $60.00
- Dual color Fluorospot plate: $90.00
- Triple color Fluorospot plate: $120.00
- Quadruple color Fluorospot plate: $150.00
- Express Service: $20.00/plate (by request, evaluation on same business day as plates are received)
- Category B plate fee: $25.00 (highly suboptimal plate conditions and demanding evaluation)
Shipping costs for return of plates/domestic: $10.00 – $16.00
Shipping costs/international: please inquire
Average turn-around time: 2-3 days
Plate storage: By request we can store plates for a limited time, for example until the end of a study. Storage costs are as follows:
- <20 plates: $ 20.00/month
- 21 to <50 plates: $50.00/month
- >50 plates: $100.00/month
Our expert consulting services cover all aspects of Elispot, including trouble shooting, review of protocol, guidance for establishing your SOP, standardization and validation procedures and guidelines, setup for clinical trial immune monitoring, certification services, evaluation of plates, training procedures, setup and performance of proficiency panels, GLP compliance, review of clinical immune monitoring data before submission, guidance in outsourcing your Elispot testing, and more.
Consulting is offered via Teams/Zoom, email, webinar, in person – when you need it, as you need it. Please inquire: .
We analyze your Elispot data for positivity. If more than one antigen are used, the analysis is done with a permutation-based method that takes into account that normal distribution of Elispot data cannot be assumed under commonly used setup conditions of the assay . The method has been shown to control the false positive rate while having superb sensitivity. For positivity testing of results from assays testing just one antigen, the appropriate tests are applied.
Operator-dependent variability of Elispot reading results is a known phenomenon, and has been described.
ZellNet offers a service to certify proficiency in Elispot plate evaluation. Such certification should be periodically pursued for each scientist in a laboratory running its own plate evaluation.
This service is independent of the Elispot reader used in-house. Simply send your plate(s) in for re-evaluation. Specify the request for certification. Also, provide your reading results and, if feasible, setup information of your plate(s), if you wish to obtain statistical analysis of the comparison of both reading results.
We will perform an extensive evaluation of the plate including auditing of all wells, spot appearance, analysis of artifacts and any problems observed (if necessary) and provide a detailed report.
You can engage one of our scientists to give a seminar at your location. You choose the topic(s), we do the rest. Examples of seminar topics are:
- Principles of the Elispot technique
- Standardization and validation procedures
- Elispot and GLP
- New Elispot applications and techniques
- Fluorescent Elispot (Fluorospot)
- Troubleshooting
- Elispot market and fields of application
- Evaluation of Elispot plates
- PBMC handling and logistics
- QA and QC measures
- Statistical analysis of Elispot results
Contact us for details
A workshop provides training for groups of 1 – 10 people. The workshop goal is to provide technical expertise:
- for cell preparation including PBMC isolation, freezing and thawing procedures
- to perform an Elispot assay
- for Elispot plate evaluation.
Workshops include:
- establishment of lab-specific protocols
- hands-on training for Elispot performance
- performance of complete Elispot experiments by participants using lab-specific protocol
- troubleshooting of typical problems encountered
- discussion of performed experiment and results
- design of a strategy for assay standardization and validation as well as for training guidelines.
Do you have an (older) Zeiss Elispot Reader?
ZellNet offers help in trouble-shooting and configuring your Zeiss Elispot Reader.
We provide training and support (hardware & software) for Zeiss Elispot Reader use.
If you don’t use your Zeiss Elispot Reader anymore, please contact us for choices including buy-back options.
