Developing a reliable multi-parametric flow cytometry panel can be very time-consuming, so any previous experience with specific markers and reagents can be valuable in reducing the time and monetary cost. Since 2010, Cytometry Part A publishes Optimized Multicolor Immunofluorescence Panels (OMIPs). Before starting to develop a new panel, it is worth checking if someone has published a panel that could potentially be adapted to your needs at the dedicated Cytometry Part A section.
For online trainings and webinars on specific flow cytometry techniques you can also visit Cyto U, ISAC’s on-line portal for cytometry education.
Resulting from a large international inter-laboratory effort spear-headed by the Cancer Immunotherapy Consortium, harmonization guidelines for Gating have been published and recently confirmed in a second gating panel.
