New: Book on the Ins and Outs of Elispot published
The book “Elispot for Rookies (and Experts too)” has just been published. It contains all information on Elispot one needs to know in order to run a high quality assay. The book also contains detailed information on sample preparation, plate evaluation and statistical analysis of results. It is available through Springer, Barnes and Noble as well as Amazon.
Elispot Webinar June 15th, 2:30 pm EDT
A free Elispot webinar will take place on Wednesday, June 15th, 2:30 pm EDT. the topic: “Not All Spots are Created Equal: Maximizing Data Quality in ELISpot Bio-Assays Through Stepwise Process Validation“. Register here.
Press release: Partnership with Medgenome
ZellNet is excited about the opportunity to expand its offered services with MedGenome’s cutting-edge OncoPept™ platform, a logical and timely extension. The partnership allows us to integrate important steps within the translational research and vaccine development pipeline: from what to monitor to how to monitor. Customers can continue relying on ZellNet’s superior service standards. We are proud to partner with such an exceptional company as MedGenome, and look forward to passing the advantages of this high value addition to our customers”. Read the Press Release (June 4,2016).
MIATA Hall of Fame filling up!
Reporting sufficient details in scientific publications has been a focus of many recent activities including NIH workshops. We proudly report that the number of papers that report in compliance with the MIATA guidelines (Minimal information about T-cell assays) is constantly growing. Check out the MIATA Hall of Fame where you can find papers with detailed reporting on Elispot and other immune monitoring assays.
Immune monitoring technology primer published in JITC
As part of the activities of the SITC Biomarker Taskforce, an immune monitoring technology primer on Elispot and Fluorospot has just been published in the Journal of Immunotherapy of Cancer (JITC). It gives an overview of the current status of the assay and its applicability for immune monitoring and biomarker work. The article can be freely accessed at the JITC website.
Elispot Plate Reading Guidelines published in Nature Protocols
The results of the Elispot harmonization efforts of the Cancer Immunotherapy Consortium (CIC/CRI), in collaboration with European Association of Immunotherapy of Cancer (CIMT), have led to guidelines for the evaluation of Elispot plates, based on a large Elispot plate reading panel and consensus process involving more than 100 scientists from all over the world. The guidelines have recently been published in Nature Protocols.
Another cause for Overnight Resting
A group from TPIMS in San Diego revisits the effects of overnight resting on frozen PBMC and their performance in IFNg Elispot. The advantages of including a resting step is clearly demonstrated and confirmed by rigorous statistical testing, using an adapted version of the DFR method for comparing multiple test runs of the same sample.
The Know-How on FluoroSpot Evaluation
The advantages of the multi-level evaluation approach of FluoroSpot plates is being explained and demonstrated in detail in this new publication, elevating the single cytokine Elispot technique to a poly-functional monitoring tool.
Excellent publication about IVS
Fresh of the press comes an excellent publication from Chudley et al. about in vitro stimulation of PBMC, harmonization of IVS protocols, and effects of IVS on results from Elispot and Multimer assays. This paper can be found in Cancer Immunology and Immunotherapy. It is written in full MIATA compliance and hence also listed in the MIATA Hall of Fame.
Outstanding publication on the effect of Overnight resting on previously frozen PBMC
Please check out this excellent publication in PLOS One on the effects of overnight resting on PBMC in the immune monitoring setting. Note that overnight resting has been recommended in the Elispot harmonization guidelines published in CII in 2008, based on results of large proficiency panel testing projects.